Description:
AssayKitFeatures&Benefits:
TheLyraAdenovirusAssayisareal-timePCRinvitrodiagnosticassayforthequalitativedetectionofhumanAdenovirus(HAdV)viralDNAwhichhasbeenisolatedfromnasalandnasopharyngealswabsobtainedfromindividualsexhibitingsignsandsymptomsofacuterespiratoryinfections.Thetestdetects,butdoesnotdifferentiate,HAdVspecies(A,B,C,D,E,andF)orSEROtypes.
Duringthetest,amultiplexRT-PCRreactioniscarriedoutunderoptimizedconditionsinasingletubegeneratingampliconsforeachofthetargetvirusespresentinthesample.Identificationofadenovirus(HAdV)andthePRCoccurbytheuseoftarget-specificprimersandfluorescent-labeledprobeswhichhybridizeconservedregionsinthegenomesofadenovirusandthePRC.
TheLyraAdenovirusAssayhasaconvenient,one-stepreagentsetupperformedatroomtemperatureonthebenchtop.Moreover,optimizedcyclingconditionsmakeresultsavailableinlessthan75minutesfollowingextractiontoaidintimelydiagnosisandtreatment.LyraAdenovirusRT-PCRisavailableforuseontheAppliedBiosystems®7500FastDxReal-TimePCRInstrument.
TheLyraAdenovirusAssay,whenusedwithotherLyraassays,providestheflexibilitytocustomizerespiratorypanelswithotherLyraReal-TimePCRRespiratoryassaysinordertoadapttotheshiftingdemandscausedbyvariablelocalorseasonaldiseaseprevalence.
| Feature | Benefit |
|---|---|
One-stepreagentset-up | RehydrationsolutionissimplyaddedtothelyophilizedMasterMix |
Speed | Resultsinlessthan75minutesafterextraction |
Easytraining | Simplifiedanduniformworkflowwithstandardpipettingvolumes |
Refrigeratedstorage | Nofreezerneeded.2°Cto8°Cstorage |
Longshelflife | 2yearsfromdateofmanufacture |
Roomtemperaturesetup | Noiceorcoolingblockrequired |
FlexIBLe96-testformat | Kitincludesvialsof12x8MasterMix,RehydrationSolutionandliquidProcessControls |
Multi-wellmultiplexpanels | StandardizedThermocyclingconditionsfortherespiratoryassaysallowformultipleanalytestobeanalyzedonthesameplatefromthesameextractedspecimen,effectivelyprovidingacustomizablepanelofresults |
ProductSpecifications:
| ProductSpecName | ProductSpecData |
|---|---|
Sampletype | Nasalswab,Nasopharyngealswab |
Timetoresults | Lessthan75minutesafterextraction |
Reagentstorageconditions | 2°Cto8°C |
Controlsstorageconditions | 2°Cto8°C |
Sensitivity* | 100% |
Specificity* | 95.5% |
PositivePercentAgreement* | 100% |
NegativePercentAgreement* | 98.7% |
LOD | A/31:8.00x10-2;B/3:8.00x10-2;C/1:8.00x10-2;D/19:1.61x101;E/4:1.00x100;F/41:3.20x10-2(TCID50/mL) |
Samplevolumeneededforextraction | 180µL |
Purifiednucleicacidvolumeneededtotesting | 5µL |
Shelflife | 24months |
*RefertoPackageInsertforadditionalperformanceclaims.
