Description:
QuidelMolecularInfluenzaA+BControlSetFeatures&Benefits:
TheLyraInfluenzaA+BAssayisamultiplexreal-timeRT-PCRassayforthequalitativedetectionandidentificationofviralnucleicacidsextractedfromnasalswabsand nasopharyngealswabsspecimens.Thereal-timeinfluenzatesthasbeenshowntodetectHIN1,H3N2andH7N9subtypesbutdoesnotdifferentiatethesubtypesfromInfluenzaA.
AmultiplexRT-PCRreactioniscarriedoutunderoptimizedconditionsinasingletubegeneratingampliconsforeachofthetargetvirusespresentinthesample.IdentificationofinfluenzaAoccursbytheuseoftargetspecificprimersandafluorescent-labeledprobethathybridizestoaconservedregionwithinthematrixproteingene.IdentificationofinfluenzaBoccursbytheuseoftargetspecificprimersandfluorescent-labeledprobesthathybridizetoaconservedinfluenzaBsequencewithintheneuraminidasegene.
TheLyraInfluenzaA+BAssayhasaone-stepreagentsetup.Testresultsareavailableinlessthan75minutesafterextraction.LyraInfluenzaA+BRT-PCRisavailableforuseontheAppliedBiosystems®7500FastDxReal-TimePCRInstrument,QuantStudioTMDxReal-TimePCRInstrumentandCepheid®SmartCycler®II.
TheLyraInfluenzaA+BAssayisoneinagrowingmenuofassaysandisintendedtoaidinthedifferentialdiagnosisofinfluenzaAand/orinfluenzaBviralinfectionsinhumans.
| Feature | Benefit |
|---|---|
One-stepreagentset-up | RehydrationsolutionissimplyaddedtothelyophilizedMasterMix |
Speed | Resultsinlessthan75minutesafterextraction |
Easytraining | Simplifiedanduniformworkflowwithstandardpipettingvolumes |
Refrigeratedstorage | Nofreezerneeded.2°Cto8°Cstorage |
Longshelflife | 2yearsfromdateofmanufacture |
Roomtemperaturesetup | Noiceorcoolingblockrequired |
FlexIBLe96-testformat | Kitincludesvialsof12x8MasterMix,RehydrationSolutionandliquidProcessControls |
Multi-wellmultiplexpanels | StandardizedThermocyclingconditionsfortherespiratoryassaysallowformultipleanalytestobeanalyzedonthesameplatefromthesameextractedspecimen,effectivelyprovidingacustomizablepanelofresults |
ProductSpecifications:
| ProductSpecName | ProductSpecData |
|---|---|
Sampletype | Nasalswab,Nasopharyngealswab |
Timetoresults | Lessthan75minutesafterextraction |
Reagentstorageconditions | 2°Cto8°C |
Controlsstorageconditions | 2°Cto8°C |
PositivePercentAgreement* | A:100%;B:98.4% |
NegativePercentAgreement* | A:98.7%;B:95.5% |
LOD | 2to100TCID50/mL |
Samplevolumeneededforextraction | 180µL |
Purifiednucleicacidvolumeneededtotesting | 5µL |
Shelflife | 24months |
*RefertoPackageInsertforadditionalperformanceclaims.
